Vitamin D receptor expression in mature osteoclasts reduces bone loss due to low dietary calcium intake in male mice

Y Starczak; DC Reinke; KR Barratt; PK Russell; MV Clarke; RA Davey; GJ Atkins; PH Anderson

Journal article

Vitamin D receptor expression in mature osteoclasts reduces bone loss due to low dietary calcium intake in male mice

Y Starczak, DC Reinke, KR Barratt, PK Russell, MV Clarke, RA Davey, GJ Atkins, PH Anderson

Journal of Steroid Biochemistry and Molecular Biology | Published : 2021

DOI: 10.1016/j.jsbmb.2021.105857

Abstract

Mature osteoclasts express the vitamin D receptor (VDR) and are able to respond to active vitamin D (1α, 25-dihydroxyvitamin D3; 1,25(OH)2D3) by regulating cell maturation and activity. However, the in vivo consequences of vitamin D signalling directly within functionally mature osteoclasts is only partially understood. To investigate the in vivo role of VDR in mature osteoclasts, conditional deletion of the VDR under control of the cathepsin K promoter (CtskCre/Vdr−/−), was assessed in 6 and 12-week-old mice, either under normal dietary conditions (NormCaP) or when fed a low calcium (0.03 %), low phosphorous (0.08 %) diet (LowCaP). Splenocytes from CtskCre/Vdr−/− mice were co-cultured with ..

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University of Melbourne Researchers

Michele Clarke Author

Rachel Davey Author

Related Projects (1)

CONTROL OF BONE REMODELLING BY OSTEOCLASTIC METABOLISM OF VITAMIN D

Vitamin D is essential for bone health. This study will build on our extensive body of preliminary data to investigate the extent to which b..

Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

The authors would like to acknowledge the significant contributions to this study and to the vitamin D field in general of the late Professor Howard Morris. This work was supported by Project Grant funding from the National Health and Medical Research Council of Australia (NHMRC,IDs 1029926 and 1029756) . GJA was supported by a NHMRC Senior Research Fellowship. YS and DCR were supported by postgraduate scholarships from the University of South Australia and the University of Adelaide, respectively.